Construct: shRNA TRCN0000350457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014916.2-2817s21c1
DNA Barcode:
CCCACGTGTTTAGATGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LMTK2 (22853)
Gene Description:
lemur tyrosine kinase 2
Transcript:
RefSeq NM_014916.2 (NON-CURRENT)
Match location:
Position 2817 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACGTGTTTAGATGTTATT
Hairpin Sequence:
5'-CCGG-CCCACGTGTTTAGATGTTATT-CTCGAG-AATAACATCTAAACACGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACGTGTTTAGATGTTATTCTCGAGAATAACATCTAAACACGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACGTGTTTAGATGTTATTCTCGAGAATAACATCTAAACACGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000194720