Construct: shRNA TRCN0000350469

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018486.1-552s21c1
DNA Barcode:
GCGTATTCTCTACGTGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HDAC8 (55869)
Gene Description:
histone deacetylase 8
Transcript:
RefSeq NM_018486.1 (NON-CURRENT)
Match location:
Position 552 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350469)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTATTCTCTACGTGGATTT
Hairpin Sequence:
5'-CCGG-GCGTATTCTCTACGTGGATTT-CTCGAG-AAATCCACGTAGAGAATACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTATTCTCTACGTGGATTTCTCGAGAAATCCACGTAGAGAATACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTATTCTCTACGTGGATTTCTCGAGAAATCCACGTAGAGAATACGC-3'

Other clones with same target sequence:

  1. TRCN0000004851