Construct: shRNA TRCN0000350563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010247.2-1952s21c1
DNA Barcode:
AGCTCAGAAGCCCAGCCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xrcc6 (14375)
Gene Description:
X-ray repair complementing defective repair in Chinese hamster cells 6
Transcript:
RefSeq NM_010247.2 (CURRENT)
Match location:
Position 1952 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTCAGAAGCCCAGCCACTT
Hairpin Sequence:
5'-CCGG-AGCTCAGAAGCCCAGCCACTT-CTCGAG-AAGTGGCTGGGCTTCTGAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTCAGAAGCCCAGCCACTTCTCGAGAAGTGGCTGGGCTTCTGAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTCAGAAGCCCAGCCACTTCTCGAGAAGTGGCTGGGCTTCTGAGCT-3'

Other clones with same target sequence:

  1. TRCN0000012218