Construct: shRNA TRCN0000350566

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009744.3-373s21c1
DNA Barcode:
CAACCTGAGGGAAGGCAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl6 (12053)
Gene Description:
B cell leukemia/lymphoma 6
Transcript:
RefSeq NM_009744.3 (NON-CURRENT)
Match location:
Position 373 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350566)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCTGAGGGAAGGCAATAT
Hairpin Sequence:
5'-CCGG-CAACCTGAGGGAAGGCAATAT-CTCGAG-ATATTGCCTTCCCTCAGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCTGAGGGAAGGCAATATCTCGAGATATTGCCTTCCCTCAGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCTGAGGGAAGGCAATATCTCGAGATATTGCCTTCCCTCAGGTTG-3'

Other clones with same target sequence:

(none)