Construct: shRNA TRCN0000350573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013735.3-6516s21c1
DNA Barcode:
TGAATGGACAGTGACTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trp53bp1 (27223)
Gene Description:
transformation related protein 53 binding protein 1
Transcript:
RefSeq NM_013735.3 (NON-CURRENT)
Match location:
Position 6516 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAATGGACAGTGACTATAAA
Hairpin Sequence:
5'-CCGG-TGAATGGACAGTGACTATAAA-CTCGAG-TTTATAGTCACTGTCCATTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAATGGACAGTGACTATAAACTCGAGTTTATAGTCACTGTCCATTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAATGGACAGTGACTATAAACTCGAGTTTATAGTCACTGTCCATTCA-3'

Other clones with same target sequence:

(none)