Construct: shRNA TRCN0000350656

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152825.2-902s21c1
DNA Barcode:
CAGAAGGCTCCTCGGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp45 (77593)
Gene Description:
ubiquitin specific petidase 45
Transcript:
RefSeq NM_152825.2 (CURRENT)
Match location:
Position 902 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350656)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGGCTCCTCGGTTTAAA
Hairpin Sequence:
5'-CCGG-CAGAAGGCTCCTCGGTTTAAA-CTCGAG-TTTAAACCGAGGAGCCTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGGCTCCTCGGTTTAAACTCGAGTTTAAACCGAGGAGCCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGGCTCCTCGGTTTAAACTCGAGTTTAAACCGAGGAGCCTTCTG-3'

Other clones with same target sequence:

(none)