Construct: shRNA TRCN0000350685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023058.3-1245s21c1
DNA Barcode:
GAACTGCTGCAGGGCTCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkmyt1 (268930)
Gene Description:
protein kinase, membrane associated tyrosine/threonine 1
Transcript:
RefSeq NM_023058.3 (CURRENT)
Match location:
Position 1245 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTGCTGCAGGGCTCTTAT
Hairpin Sequence:
5'-CCGG-GAACTGCTGCAGGGCTCTTAT-CTCGAG-ATAAGAGCCCTGCAGCAGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTGCTGCAGGGCTCTTATCTCGAGATAAGAGCCCTGCAGCAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTGCTGCAGGGCTCTTATCTCGAGATAAGAGCCCTGCAGCAGTTC-3'

Other clones with same target sequence:

(none)