Construct: shRNA TRCN0000350720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011876.3-620s21c1
DNA Barcode:
GATCCGAATCAATGAGGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Twf2 (23999)
Gene Description:
twinfilin actin binding protein 2
Transcript:
RefSeq NM_011876.3 (CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCGAATCAATGAGGTGAA
Hairpin Sequence:
5'-CCGG-GATCCGAATCAATGAGGTGAA-CTCGAG-TTCACCTCATTGATTCGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCGAATCAATGAGGTGAACTCGAGTTCACCTCATTGATTCGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCGAATCAATGAGGTGAACTCGAGTTCACCTCATTGATTCGGATC-3'

Other clones with same target sequence:

  1. TRCN0000023885