Construct: shRNA TRCN0000350733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145931.2-1378s21c1
DNA Barcode:
TATGAATGGACCAGGTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zc3h7a (106205)
Gene Description:
zinc finger CCCH type containing 7 A
Transcript:
RefSeq NM_145931.2 (CURRENT)
Match location:
Position 1378 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGAATGGACCAGGTAATTT
Hairpin Sequence:
5'-CCGG-TATGAATGGACCAGGTAATTT-CTCGAG-AAATTACCTGGTCCATTCATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGAATGGACCAGGTAATTTCTCGAGAAATTACCTGGTCCATTCATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGAATGGACCAGGTAATTTCTCGAGAAATTACCTGGTCCATTCATA-3'

Other clones with same target sequence:

(none)