Construct: shRNA TRCN0000350793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145442.1-1084s21c1
DNA Barcode:
TGCTGTTTCTAGTCGTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POTEM (641455)
Gene Description:
POTE ankyrin domain family member M
Transcript:
RefSeq NM_001145442.1 (CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGTTTCTAGTCGTCATAA
Hairpin Sequence:
5'-CCGG-TGCTGTTTCTAGTCGTCATAA-CTCGAG-TTATGACGACTAGAAACAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGTTTCTAGTCGTCATAACTCGAGTTATGACGACTAGAAACAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGTTTCTAGTCGTCATAACTCGAGTTATGACGACTAGAAACAGCA-3'

Other clones with same target sequence:

(none)