Construct: shRNA TRCN0000350822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152432.2-945s21c1
DNA Barcode:
TGTTACTAGCTCACCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP42 (143872)
Gene Description:
Rho GTPase activating protein 42
Transcript:
RefSeq NM_152432.2 (NON-CURRENT)
Match location:
Position 945 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTACTAGCTCACCGGAAAT
Hairpin Sequence:
5'-CCGG-TGTTACTAGCTCACCGGAAAT-CTCGAG-ATTTCCGGTGAGCTAGTAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTACTAGCTCACCGGAAATCTCGAGATTTCCGGTGAGCTAGTAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTACTAGCTCACCGGAAATCTCGAGATTTCCGGTGAGCTAGTAACA-3'

Other clones with same target sequence:

(none)