Construct: shRNA TRCN0000350850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001143987.1-1130s21c1
DNA Barcode:
GGCTAATCCAGGACACCATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBPF6 (653149)
Gene Description:
NBPF member 6
Transcript:
RefSeq NM_001143987.1 (CURRENT)
Match location:
Position 1130 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTAATCCAGGACACCATGA
Hairpin Sequence:
5'-CCGG-GGCTAATCCAGGACACCATGA-CTCGAG-TCATGGTGTCCTGGATTAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTAATCCAGGACACCATGACTCGAGTCATGGTGTCCTGGATTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTAATCCAGGACACCATGACTCGAGTCATGGTGTCCTGGATTAGCC-3'

Other clones with same target sequence:

(none)