Construct: shRNA TRCN0000350860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145045.1-1976s21c1
DNA Barcode:
GGTTCTTTATGTCCGTTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF286B (729288)
Gene Description:
zinc finger protein 286B (pseudogene)
Transcript:
RefSeq NM_001145045.1 (NON-CURRENT)
Match location:
Position 1976 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTCTTTATGTCCGTTAATT
Hairpin Sequence:
5'-CCGG-GGTTCTTTATGTCCGTTAATT-CTCGAG-AATTAACGGACATAAAGAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTCTTTATGTCCGTTAATTCTCGAGAATTAACGGACATAAAGAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTCTTTATGTCCGTTAATTCTCGAGAATTAACGGACATAAAGAACC-3'

Other clones with same target sequence:

(none)