Construct: shRNA TRCN0000350906

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024046.3-382s21c1
DNA Barcode:
GCGGAAAGCTGCCAAGAACGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMKV (79012)
Gene Description:
CaM kinase like vesicle associated
Transcript:
RefSeq NM_024046.3 (NON-CURRENT)
Match location:
Position 382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350906)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAAAGCTGCCAAGAACGA
Hairpin Sequence:
5'-CCGG-GCGGAAAGCTGCCAAGAACGA-CTCGAG-TCGTTCTTGGCAGCTTTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAAAGCTGCCAAGAACGACTCGAGTCGTTCTTGGCAGCTTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAAAGCTGCCAAGAACGACTCGAGTCGTTCTTGGCAGCTTTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000010254