Construct: shRNA TRCN0000350958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053252.3-3250s21c1
DNA Barcode:
GAGGGTGGGACTTAGCTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehbp1l1 (114601)
Gene Description:
EH domain binding protein 1-like 1
Transcript:
RefSeq NM_053252.3 (CURRENT)
Match location:
Position 3250 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGGTGGGACTTAGCTAATA
Hairpin Sequence:
5'-CCGG-GAGGGTGGGACTTAGCTAATA-CTCGAG-TATTAGCTAAGTCCCACCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGGTGGGACTTAGCTAATACTCGAGTATTAGCTAAGTCCCACCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGGTGGGACTTAGCTAATACTCGAGTATTAGCTAAGTCCCACCCTC-3'

Other clones with same target sequence:

(none)