Construct: shRNA TRCN0000350960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018824.2-1513s21c1
DNA Barcode:
CTGTGTTCTTGATGGCATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc23a2 (54338)
Gene Description:
solute carrier family 23 (nucleobase transporters), member 2
Transcript:
RefSeq NM_018824.2 (CURRENT)
Match location:
Position 1513 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGTTCTTGATGGCATATT
Hairpin Sequence:
5'-CCGG-CTGTGTTCTTGATGGCATATT-CTCGAG-AATATGCCATCAAGAACACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGTTCTTGATGGCATATTCTCGAGAATATGCCATCAAGAACACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGTTCTTGATGGCATATTCTCGAGAATATGCCATCAAGAACACAG-3'

Other clones with same target sequence:

  1. TRCN0000370606