Construct: shRNA TRCN0000350972

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005869.2-1665s21c1
DNA Barcode:
GCTGGAAATGTGCCTACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CWC27 (10283)
Gene Description:
CWC27 spliceosome associated cyclophilin
Transcript:
RefSeq NM_005869.2 (NON-CURRENT)
Match location:
Position 1665 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000350972)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAAATGTGCCTACAATG
Hairpin Sequence:
5'-CCGG-GCTGGAAATGTGCCTACAATG-CTCGAG-CATTGTAGGCACATTTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAAATGTGCCTACAATGCTCGAGCATTGTAGGCACATTTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAAATGTGCCTACAATGCTCGAGCATTGTAGGCACATTTCCAGC-3'

Other clones with same target sequence:

(none)