Construct: shRNA TRCN0000351057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022814.2-3428s21c1
DNA Barcode:
TCGTGTCCGCTGGGTACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Svep1 (64817)
Gene Description:
sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1
Transcript:
RefSeq NM_022814.2 (CURRENT)
Match location:
Position 3428 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGTGTCCGCTGGGTACTTAT
Hairpin Sequence:
5'-CCGG-TCGTGTCCGCTGGGTACTTAT-CTCGAG-ATAAGTACCCAGCGGACACGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGTGTCCGCTGGGTACTTATCTCGAGATAAGTACCCAGCGGACACGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGTGTCCGCTGGGTACTTATCTCGAGATAAGTACCCAGCGGACACGA-3'

Other clones with same target sequence:

(none)