Construct: shRNA TRCN0000351308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013657.5-785s21c1
DNA Barcode:
CCGAAGTTTAACTAAGAGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema3c (20348)
Gene Description:
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Transcript:
RefSeq NM_013657.5 (CURRENT)
Match location:
Position 785 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGTTTAACTAAGAGGAA
Hairpin Sequence:
5'-CCGG-CCGAAGTTTAACTAAGAGGAA-CTCGAG-TTCCTCTTAGTTAAACTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGTTTAACTAAGAGGAACTCGAGTTCCTCTTAGTTAAACTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGTTTAACTAAGAGGAACTCGAGTTCCTCTTAGTTAAACTTCGG-3'

Other clones with same target sequence:

  1. TRCN0000067389