Construct: shRNA TRCN0000351415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177992.2-1120s21c2
DNA Barcode:
GCTGCTAAACTGAAGGAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gmpr2 (105446)
Gene Description:
guanosine monophosphate reductase 2
Transcript:
RefSeq NM_177992.2 (CURRENT)
Match location:
Position 1120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTAAACTGAAGGAACTA
Hairpin Sequence:
5'-CCGG-GCTGCTAAACTGAAGGAACTA-CTCGAG-TAGTTCCTTCAGTTTAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTAAACTGAAGGAACTACTCGAGTAGTTCCTTCAGTTTAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTAAACTGAAGGAACTACTCGAGTAGTTCCTTCAGTTTAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000042168