Construct: shRNA TRCN0000351447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177992.2-550s21c3
DNA Barcode:
GCTAACGGCTACTCTGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gmpr2 (105446)
Gene Description:
guanosine monophosphate reductase 2
Transcript:
RefSeq NM_177992.2 (CURRENT)
Match location:
Position 550 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACGGCTACTCTGAACAT
Hairpin Sequence:
5'-CCGG-GCTAACGGCTACTCTGAACAT-CTCGAG-ATGTTCAGAGTAGCCGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACGGCTACTCTGAACATCTCGAGATGTTCAGAGTAGCCGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACGGCTACTCTGAACATCTCGAGATGTTCAGAGTAGCCGTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000042172