Construct: shRNA TRCN0000351484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146150.2-3698s21c2
DNA Barcode:
CCAAACACTTACGTCAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nrd1 (230598)
Gene Description:
nardilysin, N-arginine dibasic convertase, NRD convertase 1
Transcript:
RefSeq NM_146150.2 (CURRENT)
Match location:
Position 3698 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACACTTACGTCAGCAAT
Hairpin Sequence:
5'-CCGG-CCAAACACTTACGTCAGCAAT-CTCGAG-ATTGCTGACGTAAGTGTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACACTTACGTCAGCAATCTCGAGATTGCTGACGTAAGTGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACACTTACGTCAGCAATCTCGAGATTGCTGACGTAAGTGTTTGG-3'

Other clones with same target sequence:

  1. TRCN0000031559