Construct: shRNA TRCN0000351614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024106.1-514s21c2
DNA Barcode:
CAGATCATCAAACCCAGTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF426 (79088)
Gene Description:
zinc finger protein 426
Transcript:
RefSeq NM_024106.1 (NON-CURRENT)
Match location:
Position 514 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATCATCAAACCCAGTCTA
Hairpin Sequence:
5'-CCGG-CAGATCATCAAACCCAGTCTA-CTCGAG-TAGACTGGGTTTGATGATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATCATCAAACCCAGTCTACTCGAGTAGACTGGGTTTGATGATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATCATCAAACCCAGTCTACTCGAGTAGACTGGGTTTGATGATCTG-3'

Other clones with same target sequence:

  1. TRCN0000016726