Construct: shRNA TRCN0000351669

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133834.1-395s21c2
DNA Barcode:
GCAGGTGTTCATTTCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hnrnpf (98758)
Gene Description:
heterogeneous nuclear ribonucleoprotein F
Transcript:
RefSeq NM_133834.1 (NON-CURRENT)
Match location:
Position 395 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351669)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTGTTCATTTCATTTAT
Hairpin Sequence:
5'-CCGG-GCAGGTGTTCATTTCATTTAT-CTCGAG-ATAAATGAAATGAACACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTGTTCATTTCATTTATCTCGAGATAAATGAAATGAACACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTGTTCATTTCATTTATCTCGAGATAAATGAAATGAACACCTGC-3'

Other clones with same target sequence:

  1. TRCN0000109261