Construct: shRNA TRCN0000351840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175771.4-502s21c1
DNA Barcode:
GCATTCCTGGTGGGTTTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem47 (192216)
Gene Description:
transmembrane protein 47
Transcript:
RefSeq NM_175771.4 (NON-CURRENT)
Match location:
Position 502 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCCTGGTGGGTTTGATT
Hairpin Sequence:
5'-CCGG-GCATTCCTGGTGGGTTTGATT-CTCGAG-AATCAAACCCACCAGGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCCTGGTGGGTTTGATTCTCGAGAATCAAACCCACCAGGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCCTGGTGGGTTTGATTCTCGAGAATCAAACCCACCAGGAATGC-3'

Other clones with same target sequence:

  1. TRCN0000157498
  2. TRCN0000126230