Construct: shRNA TRCN0000351884

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029494.2-1466s21c1
DNA Barcode:
GCAGTCTTCTTCAGTCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab30 (75985)
Gene Description:
RAB30, member RAS oncogene family
Transcript:
RefSeq NM_029494.2 (CURRENT)
Match location:
Position 1466 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351884)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCTTCTTCAGTCTCATT
Hairpin Sequence:
5'-CCGG-GCAGTCTTCTTCAGTCTCATT-CTCGAG-AATGAGACTGAAGAAGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCTTCTTCAGTCTCATTCTCGAGAATGAGACTGAAGAAGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCTTCTTCAGTCTCATTCTCGAGAATGAGACTGAAGAAGACTGC-3'

Other clones with same target sequence:

  1. TRCN0000100400