Construct: shRNA TRCN0000351895

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007754.2-775s21c1
DNA Barcode:
CGCAGGAACAAGTTTGTGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpd (12874)
Gene Description:
carboxypeptidase D
Transcript:
RefSeq NM_007754.2 (CURRENT)
Match location:
Position 775 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000351895)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGGAACAAGTTTGTGCTT
Hairpin Sequence:
5'-CCGG-CGCAGGAACAAGTTTGTGCTT-CTCGAG-AAGCACAAACTTGTTCCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGGAACAAGTTTGTGCTTCTCGAGAAGCACAAACTTGTTCCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGGAACAAGTTTGTGCTTCTCGAGAAGCACAAACTTGTTCCTGCG-3'

Other clones with same target sequence:

  1. TRCN0000222511