Construct: shRNA TRCN0000352049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130861.2-2271s21c1
DNA Barcode:
GCTCACTCATTCTAGTCACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slco1a5 (108096)
Gene Description:
solute carrier organic anion transporter family, member 1a5
Transcript:
RefSeq NM_130861.2 (NON-CURRENT)
Match location:
Position 2271 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTCATTCTAGTCACAT
Hairpin Sequence:
5'-CCGG-GCTCACTCATTCTAGTCACAT-CTCGAG-ATGTGACTAGAATGAGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTCATTCTAGTCACATCTCGAGATGTGACTAGAATGAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTCATTCTAGTCACATCTCGAGATGTGACTAGAATGAGTGAGC-3'

Other clones with same target sequence:

  1. TRCN0000079238