Construct: shRNA TRCN0000352093

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178651.3-286s21c1
DNA Barcode:
CCCGACATTCACCCACATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc30a9 (109108)
Gene Description:
solute carrier family 30 (zinc transporter), member 9
Transcript:
RefSeq NM_178651.3 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352093)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGACATTCACCCACATTTA
Hairpin Sequence:
5'-CCGG-CCCGACATTCACCCACATTTA-CTCGAG-TAAATGTGGGTGAATGTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGACATTCACCCACATTTACTCGAGTAAATGTGGGTGAATGTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGACATTCACCCACATTTACTCGAGTAAATGTGGGTGAATGTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000079695