Construct: shRNA TRCN0000352182
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011288.1-109s21c1
- DNA Barcode:
- GCTCCGTGTATTTCGAACTAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mrpl23 (19935)
- Gene Description:
- mitochondrial ribosomal protein L23
- Transcript:
- RefSeq NM_011288.1 (CURRENT)
- Match location:
- Position 109 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000352182)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCCGTGTATTTCGAACTAA
- Hairpin Sequence:
-
5'-CCGG-GCTCCGTGTATTTCGAACTAA-CTCGAG-TTAGTTCGAAATACACGGAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCCGTGTATTTCGAACTAACTCGAGTTAGTTCGAAATACACGGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCCGTGTATTTCGAACTAACTCGAGTTAGTTCGAAATACACGGAGC-3'
Other clones with same target sequence:
-
TRCN0000104431
