Construct: shRNA TRCN0000352251

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008875.4-3099s21c1
DNA Barcode:
CCCAATGATGAAGTACACAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pld1 (18805)
Gene Description:
phospholipase D1
Transcript:
RefSeq NM_008875.4 (CURRENT)
Match location:
Position 3099 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352251)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGATGAAGTACACAAT
Hairpin Sequence:
5'-CCGG-CCCAATGATGAAGTACACAAT-CTCGAG-ATTGTGTACTTCATCATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGATGAAGTACACAATCTCGAGATTGTGTACTTCATCATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGATGAAGTACACAATCTCGAGATTGTGTACTTCATCATTGGG-3'

Other clones with same target sequence:

  1. TRCN0000076820