Construct: shRNA TRCN0000352352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042758.1-2541s21c1
DNA Barcode:
GGGTCATGTAGCCGATATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRGAP2 (23380)
Gene Description:
SLIT-ROBO Rho GTPase activating protein 2
Transcript:
RefSeq NM_001042758.1 (NON-CURRENT)
Match location:
Position 2541 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGTCATGTAGCCGATATTTA
Hairpin Sequence:
5'-CCGG-GGGTCATGTAGCCGATATTTA-CTCGAG-TAAATATCGGCTACATGACCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGTCATGTAGCCGATATTTACTCGAGTAAATATCGGCTACATGACCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGTCATGTAGCCGATATTTACTCGAGTAAATATCGGCTACATGACCC-3'

Other clones with same target sequence:

(none)