Construct: shRNA TRCN0000352404

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145063.1-739s21c1
DNA Barcode:
CAGCAGAGGTTTCCTAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GATSL1 (389523)
Gene Description:
GATS protein-like 1
Transcript:
RefSeq NM_001145063.1 (NON-CURRENT)
Match location:
Position 739 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352404)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAGAGGTTTCCTAGTAAC
Hairpin Sequence:
5'-CCGG-CAGCAGAGGTTTCCTAGTAAC-CTCGAG-GTTACTAGGAAACCTCTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAGAGGTTTCCTAGTAACCTCGAGGTTACTAGGAAACCTCTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAGAGGTTTCCTAGTAACCTCGAGGTTACTAGGAAACCTCTGCTG-3'

Other clones with same target sequence:

(none)