Construct: shRNA TRCN0000352450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019840.2-2140s21c1
DNA Barcode:
CAACCGGATGCTCAAGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pde4b (18578)
Gene Description:
phosphodiesterase 4B, cAMP specific
Transcript:
RefSeq NM_019840.2 (CURRENT)
Match location:
Position 2140 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGGATGCTCAAGATATT
Hairpin Sequence:
5'-CCGG-CAACCGGATGCTCAAGATATT-CTCGAG-AATATCTTGAGCATCCGGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGGATGCTCAAGATATTCTCGAGAATATCTTGAGCATCCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGGATGCTCAAGATATTCTCGAGAATATCTTGAGCATCCGGTTG-3'

Other clones with same target sequence:

(none)