Construct: shRNA TRCN0000352609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033360.2-1273s21c1
DNA Barcode:
CAGTTGAGACCTTCTAATTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRAS (3845)
Gene Description:
KRAS proto-oncogene, GTPase
Transcript:
RefSeq NM_033360.2 (NON-CURRENT)
Match location:
Position 1273 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTGAGACCTTCTAATTGG
Hairpin Sequence:
5'-CCGG-CAGTTGAGACCTTCTAATTGG-CTCGAG-CCAATTAGAAGGTCTCAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTGAGACCTTCTAATTGGCTCGAGCCAATTAGAAGGTCTCAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTGAGACCTTCTAATTGGCTCGAGCCAATTAGAAGGTCTCAACTG-3'

Other clones with same target sequence:

  1. TRCN0000010369