Construct: shRNA TRCN0000352621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003718.3-2146s21c1
DNA Barcode:
GCTGATAGCTTACGAGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK13 (8621)
Gene Description:
cyclin dependent kinase 13
Transcript:
RefSeq NM_003718.3 (NON-CURRENT)
Match location:
Position 2146 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATAGCTTACGAGGAAAT
Hairpin Sequence:
5'-CCGG-GCTGATAGCTTACGAGGAAAT-CTCGAG-ATTTCCTCGTAAGCTATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATAGCTTACGAGGAAATCTCGAGATTTCCTCGTAAGCTATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATAGCTTACGAGGAAATCTCGAGATTTCCTCGTAAGCTATCAGC-3'

Other clones with same target sequence:

  1. TRCN0000196896