Construct: shRNA TRCN0000352685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021913.3-2335s21c1
DNA Barcode:
GATTGCCATTGAGAGTCTAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AXL (558)
Gene Description:
AXL receptor tyrosine kinase
Transcript:
RefSeq NM_021913.3 (NON-CURRENT)
Match location:
Position 2335 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTGCCATTGAGAGTCTAGC
Hairpin Sequence:
5'-CCGG-GATTGCCATTGAGAGTCTAGC-CTCGAG-GCTAGACTCTCAATGGCAATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTGCCATTGAGAGTCTAGCCTCGAGGCTAGACTCTCAATGGCAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTGCCATTGAGAGTCTAGCCTCGAGGCTAGACTCTCAATGGCAATC-3'

Other clones with same target sequence:

  1. TRCN0000196945