Construct: shRNA TRCN0000352793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001992.3-1247s21c1
DNA Barcode:
CCCACAAACGTCCTCCTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
F2R (2149)
Gene Description:
coagulation factor II thrombin receptor
Transcript:
RefSeq NM_001992.3 (NON-CURRENT)
Match location:
Position 1247 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACAAACGTCCTCCTGATT
Hairpin Sequence:
5'-CCGG-CCCACAAACGTCCTCCTGATT-CTCGAG-AATCAGGAGGACGTTTGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACAAACGTCCTCCTGATTCTCGAGAATCAGGAGGACGTTTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACAAACGTCCTCCTGATTCTCGAGAATCAGGAGGACGTTTGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000003687