Construct: shRNA TRCN0000352888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013265.2-2077s21c1
DNA Barcode:
GAAGCTATTCTCTGAACGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS51 (738)
Gene Description:
VPS51 subunit of GARP complex
Transcript:
RefSeq NM_013265.2 (NON-CURRENT)
Match location:
Position 2077 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTATTCTCTGAACGTAT
Hairpin Sequence:
5'-CCGG-GAAGCTATTCTCTGAACGTAT-CTCGAG-ATACGTTCAGAGAATAGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTATTCTCTGAACGTATCTCGAGATACGTTCAGAGAATAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTATTCTCTGAACGTATCTCGAGATACGTTCAGAGAATAGCTTC-3'

Other clones with same target sequence:

  1. TRCN0000151062