Construct: shRNA TRCN0000352947

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021215.3-1099s21c1
DNA Barcode:
GCATGTCTGTTACTAGCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPRD1B (58490)
Gene Description:
regulation of nuclear pre-mRNA domain containing 1B
Transcript:
RefSeq NM_021215.3 (NON-CURRENT)
Match location:
Position 1099 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352947)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTCTGTTACTAGCAGAA
Hairpin Sequence:
5'-CCGG-GCATGTCTGTTACTAGCAGAA-CTCGAG-TTCTGCTAGTAACAGACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTCTGTTACTAGCAGAACTCGAGTTCTGCTAGTAACAGACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTCTGTTACTAGCAGAACTCGAGTTCTGCTAGTAACAGACATGC-3'

Other clones with same target sequence:

  1. TRCN0000130891