Construct: shRNA TRCN0000352971

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006455.2-2048s21c1
DNA Barcode:
GATCACTTGAGGTCAGGAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
P3H4 (10609)
Gene Description:
prolyl 3-hydroxylase family member 4 (inactive)
Transcript:
RefSeq NM_006455.2 (NON-CURRENT)
Match location:
Position 2048 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352971)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACTTGAGGTCAGGAGTT
Hairpin Sequence:
5'-CCGG-GATCACTTGAGGTCAGGAGTT-CTCGAG-AACTCCTGACCTCAAGTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACTTGAGGTCAGGAGTTCTCGAGAACTCCTGACCTCAAGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACTTGAGGTCAGGAGTTCTCGAGAACTCCTGACCTCAAGTGATC-3'

Other clones with same target sequence:

  1. TRCN0000140719
  2. TRCN0000165299