Construct: shRNA TRCN0000352975

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018004.1-1001s21c1
DNA Barcode:
GCAGTAACCATTGTCATCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TMEM45A (55076)
Gene Description:
transmembrane protein 45A
Transcript:
RefSeq NM_018004.1 (NON-CURRENT)
Match location:
Position 1001 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000352975)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAACCATTGTCATCGTT
Hairpin Sequence:
5'-CCGG-GCAGTAACCATTGTCATCGTT-CTCGAG-AACGATGACAATGGTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAACCATTGTCATCGTTCTCGAGAACGATGACAATGGTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAACCATTGTCATCGTTCTCGAGAACGATGACAATGGTTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000130366