Construct: shRNA TRCN0000353030

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025137.3-2054s21c1
DNA Barcode:
CCCAAAGTAAAGGAGAGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPG11 (80208)
Gene Description:
SPG11 vesicle trafficking associated, spatacsin
Transcript:
RefSeq NM_025137.3 (NON-CURRENT)
Match location:
Position 2054 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353030)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAAGTAAAGGAGAGCAAT
Hairpin Sequence:
5'-CCGG-CCCAAAGTAAAGGAGAGCAAT-CTCGAG-ATTGCTCTCCTTTACTTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAAGTAAAGGAGAGCAATCTCGAGATTGCTCTCCTTTACTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAAGTAAAGGAGAGCAATCTCGAGATTGCTCTCCTTTACTTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000148925