Construct: shRNA TRCN0000353074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001552.2-1493s21c1
DNA Barcode:
TATTCTGACTTCCTCTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGFBP4 (3487)
Gene Description:
insulin like growth factor binding protein 4
Transcript:
RefSeq NM_001552.2 (NON-CURRENT)
Match location:
Position 1493 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATTCTGACTTCCTCTGATTT
Hairpin Sequence:
5'-CCGG-TATTCTGACTTCCTCTGATTT-CTCGAG-AAATCAGAGGAAGTCAGAATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATTCTGACTTCCTCTGATTTCTCGAGAAATCAGAGGAAGTCAGAATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATTCTGACTTCCTCTGATTTCTCGAGAAATCAGAGGAAGTCAGAATA-3'

Other clones with same target sequence:

(none)