Construct: shRNA TRCN0000353076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030920.3-543s21c1
DNA Barcode:
CCCGGCTTCCCAGCTTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANP32E (81611)
Gene Description:
acidic nuclear phosphoprotein 32 family member E
Transcript:
RefSeq NM_030920.3 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGGCTTCCCAGCTTAAATA
Hairpin Sequence:
5'-CCGG-CCCGGCTTCCCAGCTTAAATA-CTCGAG-TATTTAAGCTGGGAAGCCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGGCTTCCCAGCTTAAATACTCGAGTATTTAAGCTGGGAAGCCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGGCTTCCCAGCTTAAATACTCGAGTATTTAAGCTGGGAAGCCGGG-3'

Other clones with same target sequence:

(none)