Construct: shRNA TRCN0000353091

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001107.3-253s21c1
DNA Barcode:
CATCTCCAAGGTGCGTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACYP1 (97)
Gene Description:
acylphosphatase 1
Transcript:
RefSeq NM_001107.3 (NON-CURRENT)
Match location:
Position 253 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353091)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCTCCAAGGTGCGTCATAT
Hairpin Sequence:
5'-CCGG-CATCTCCAAGGTGCGTCATAT-CTCGAG-ATATGACGCACCTTGGAGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCTCCAAGGTGCGTCATATCTCGAGATATGACGCACCTTGGAGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCTCCAAGGTGCGTCATATCTCGAGATATGACGCACCTTGGAGATG-3'

Other clones with same target sequence:

(none)