Construct: shRNA TRCN0000353159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002254.6-2905s21c1
DNA Barcode:
GAAAGACCTTCCACGTCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIF3C (3797)
Gene Description:
kinesin family member 3C
Transcript:
RefSeq NM_002254.6 (NON-CURRENT)
Match location:
Position 2905 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGACCTTCCACGTCTAAA
Hairpin Sequence:
5'-CCGG-GAAAGACCTTCCACGTCTAAA-CTCGAG-TTTAGACGTGGAAGGTCTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGACCTTCCACGTCTAAACTCGAGTTTAGACGTGGAAGGTCTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGACCTTCCACGTCTAAACTCGAGTTTAGACGTGGAAGGTCTTTC-3'

Other clones with same target sequence:

(none)