Construct: shRNA TRCN0000353223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028897.3-374s21c1
DNA Barcode:
GCTCACTACTCAGCCGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mei1 (74369)
Gene Description:
meiotic double-stranded break formation protein 1
Transcript:
RefSeq NM_028897.3 (CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCACTACTCAGCCGAATAT
Hairpin Sequence:
5'-CCGG-GCTCACTACTCAGCCGAATAT-CTCGAG-ATATTCGGCTGAGTAGTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCACTACTCAGCCGAATATCTCGAGATATTCGGCTGAGTAGTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCACTACTCAGCCGAATATCTCGAGATATTCGGCTGAGTAGTGAGC-3'

Other clones with same target sequence:

(none)