Construct: shRNA TRCN0000353263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177173.5-831s21c1
DNA Barcode:
AGTGGAACTGGCGGACTAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A830018L16Rik (320492)
Gene Description:
RIKEN cDNA A830018L16 gene
Transcript:
RefSeq NM_177173.5 (NON-CURRENT)
Match location:
Position 831 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGAACTGGCGGACTAAAC
Hairpin Sequence:
5'-CCGG-AGTGGAACTGGCGGACTAAAC-CTCGAG-GTTTAGTCCGCCAGTTCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGAACTGGCGGACTAAACCTCGAGGTTTAGTCCGCCAGTTCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGAACTGGCGGACTAAACCTCGAGGTTTAGTCCGCCAGTTCCACT-3'

Other clones with same target sequence:

(none)