Construct: shRNA TRCN0000353332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016752.1-433s21c1
DNA Barcode:
CGGTAAATCCTGCAAGCCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc35b1 (110172)
Gene Description:
solute carrier family 35, member B1
Transcript:
RefSeq NM_016752.1 (CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000353332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTAAATCCTGCAAGCCAAT
Hairpin Sequence:
5'-CCGG-CGGTAAATCCTGCAAGCCAAT-CTCGAG-ATTGGCTTGCAGGATTTACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTAAATCCTGCAAGCCAATCTCGAGATTGGCTTGCAGGATTTACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTAAATCCTGCAAGCCAATCTCGAGATTGGCTTGCAGGATTTACCG-3'

Other clones with same target sequence:

  1. TRCN0000101982